INULINASE PURIFICATION PDF

Keywords: Characterization; Inulinase; Production; Purification; Streptomyces. 1. Introduction. Inulin is a polyfructan in plant consisting of linear chains. Aspergillus niger exo-inulinase purification by three phase partitioning. Inulinase (2, 1-β-D-fructan fructanohydrolase, EC ) hydrolyses inulin into. 42(4), August , pp. Purification and characterization of Inulinase from marine bacterium,. Bacillus cereus MU S Meenakshi1, S Umayaparvathi .

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Production, Purification, Characterization and Applications of Fungal Inulinases

Biochemical, biophysical properties and structure-function analysis elucidates the substrate specificity of enzyme as well as its phylogenetic evolution and relationship. Protein measurement with the folin phenol reagent. Protein content was determined by the method of Lowry et al. Inulinase activity was assayed in the reaction mixture inoculated with different enzyme concentration 0.

Results represented in Table 8 Indicated that the inulinase activity increased with increasing the substrate concentration until 1 mu and the activity was constant between 1- muinulin.

Effect of different pH values on the inulinase activity of Streptomyces grisenus. The metabolism of fructose polymers in plants. A review comparing their physiological effects. Elsevier Applied Science, London, pp: Purification, properties and comparison of invertase, exoinulinase and endoinulinase of Aspergillus ficuum.

Therefore, this study aimed to collate all information, particularly on fungal sources, characteristics, and applications of inulinases. Purification and Characterization of Exoinulinase Enzyme from Streptomyces grisenus. Effect of soluble or partly soluble dietary fibres supplementation on absorption and balance of calcium, magnesium, iron and zinc in healthy young men.

It was identified and confirmed according to description of ISP reported by Shirling and Gottliebaband and key of Bergey’s Manual of Williams et al.

Furthermore, this review highlights the various physico-chemical characteristics of fungal inulinases to determine their suitability for industrial use.

Co-operative description of type culture of Streptomyces sp. Isolation and characlerization of bacterial strains with inulinase activity. GNDuI produced high levels of extra cellular inlinase after 24 h at pH 7. Inulinases are important industrial hydrolysing enzymes which are generally used for the production of high fructose syrup and fructooligosaccharides.

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Cite this article as: Considerable evidences shows that inulinases are important industrial enzymes; however, the information on microbial sources, characteristics, and applications of inulinases is scattered in the available literature. After separation, the proteins were transferred to a inulinaxe difluoride membrane, and after staining with ponceau S solution, the inulinase protein band was cut from the membrane and subjected to amino acid sequence analysis.

Purification and characterizationof an endoinulinase from Xanthomonas oryzae No. Effect of purificatiin ions on the purified inulinase produced by Streptomyces grisenius. The analysis of the deduced inulinase amino acid sequence revealed the presence of a putative gram-positive signal peptide and a cleavage site following amino acid 39 was predicted with the program designed by Nielsen purfication al.

These results are similar to those obtained by inulinse et al. It is difficult inulinae isolate plant inulinases in sufficient quantity. The production broth medium was optimized for maximum inulinase production by addition of 0.

Pakistan Journal of Biological Sciences, 9: Highly active isolate of Streptomyces grisenus isolated from soil sample and identified according to Cause et al. Blanks were run simultaneously with the enzyme and substrate solutions. These results agreement with Abeer indicated that the activity of inulinase by streptomyces griseus increased with increasing the sub-state concentration until 1 mu. The optimum growth and inulinase activity occurred in the presence of 0. Acomparison of properties of inculinase of Fusarium oxysporum immobilised on various supports.

As shown in Table 1 the maximum activity was found on the 2nd day of incubation period.

Elution profile of the enzyme produced streptomyces grisenus after sephadex G filtration. Optimization of culture condition for inulinase production: Therefore, microbial inulinases which can be induced by growing microorganisms, have a potential for industrial use in the production of fructose frminulin Edelman and Jefford, Effect of incubation period on the inulinase activity of Streptomyces grisenus.

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Production, Purification, Characterization and Applications of Fungal Inulinases | BenthamScience

Thermal stability of inulinase immobilized on DEAE cellulose with or without purificatiom. Inulinase was purified wit a recovery of 2. Many micro organisms, including filamentous fungi, yeast, bacteria and streptomyces sp. Production of inulotriose from inulin by Inulin-degrading enzyme from Streptomyces rochei E Similar result was obtained by Kim and Byum who stated that the immobilization of inulinase on various matrices such as, nylon and 2-aminoethyl – cellulose AE-celluloseshowed that immobilization on AE-cellulose with glutaraldehyde treatment gives good results in terms of activity and stability.

In the present review, various properties of inulin as a potent substrate for inulinase production are summarized. Microbial inulinases formentation process, properties and applications. Factors affecting purified inulinase enzyme: The purification steps of extracellular inulinase from Streptomyces grisenus. The precipitate formed was obtained by centrifugation in an ultracentrifuge at These authors concluded that inulin induces inulinase production, where as cultivation using starch, maltose and lactose as carbon source s restrains the production of this enzyme by optimizing culture conditions for maximum inulinase production, purification of streptomyces grisenius crude inulinase in cell free extracts was the aim of further purifiation in order to increase its specific enzyme activity.

The results in Table 6 and 7 revealed the effect of some physical conditions on purified enzyme produced by streptomyces grisenus viz.

Purification and characterization of levanse from Streptomyces sp.